An enzyme-linked immunosorbent assay (ELISA) was used to investigate the identification of HIV using an ELISA assay. People may unknowingly be carrying the HIV virus unless their blood is tested using an ELISA assay. The test identifies if they carry the Immunoglobulin G within their serum (IgG). IgG is a specific type of antibody, directed towards the viral HIV antigen. The goal of the ELISA assay was to detect specific HIV antibody proteins which indicate a positive presence of the HIV disease. The ELISA assay technique uses a microtiter plate with 96 wells and specific IgG proteins for detection. Each of these wells have a special surface that binds strongly with proteins. The enzyme that is linked to the secondary antibody facilitates a chemical reaction that changes the color of the assay. The results of our experiment showed a color change which indicated that the patient possesses antibodies to the antigen and has tested positive for HIV. If a no change in color had occurred, that would indicate that the patient would be negative for HIV. Positive results were determined by ELISA assay during the clinical investigation. It was concluded that the antigenic ELISA immunoassay can be positivity adapted to successfully detect samples of serum that contain IgG antigens for HIV.